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Fast and affordable way to test drinking water for deadly E. coli

Researchers at the University of Waterloo have invented a fast, affordable way for developing communities to test their drinking water for potentially deadly E. coli. Unlike current tests that cost about $70 and can take up to three days to get back from the lab, the Waterloo invention uses paper strips similar to those in litmus tests to produce results in less than three hours at a cost of 50 cents.

E.coli
Low-temperature electron micrograph of a cluster of E. coli bacteria, magnified 10,000 times. Each individual bacterium is oblong shaped (Photo by Eric Erbe/Wikimedia).

“This has the potential to allow routine, affordable water testing to help billions of people in the developing world avoid getting sick,” said Sushanta Mitra, executive director of the Waterloo Institute for Nanotechnology. “It is a breakthrough.”

Now being refined by Glacierclean Technologies Inc., a startup company co-founded by Mitra, the test could also improve water safety in remote or rural areas of the developed world and greatly reduce testing costs for municipal treatment systems.

Researchers targeted E. coli — the culprit in a deadly outbreak in Walkerton, Ontario in 2000 — because it is an indicator organism of water contamination.

The bottom of the paper strip developed by Mitra and his colleagues is laced with sugar, which begins to dissolve when placed in water. E. coli bacteria are attracted by the resulting sugar trail and get trapped in the porous paper when they come in contact with it.

As water enters the paper, it carries the trapped bacteria into an area of the strip containing a mixture of chemicals. The E. colireact with those chemicals and turn the strip pinkish red to signify a positive test.

With high levels of contamination, a result is produced in just 30 minutes. Low levels of contamination take up to 180 minutes. Work is underway to reduce test times.

Glacierclean, which already sells mobile water kits to detect E. colifor about $5 a test, hopes to have its DipTest strips on the market within nine months.

“Simple ideas create paradigm shifts in technology and this is a simple, frugal innovation,” said Mitra, also a mechanical and mechatronics engineering professor at Waterloo.

Mitra’s research — done in collaboration with Naga Siva Kumar Gunda, a post-doctoral fellow and co-founder of Glacierclean, and former master’s student Saumyadeb Dasgupta — appears in the journal PLOS ONE.

References:

The above material provided by University of Waterloo. Note: Content may be edited for style and length.

Gunda NSK, Dasgupta S, Mitra SK. DipTest: A litmus test for E. coli detection in water. PLOS ONE, 2017; 12 (9): e0183234 DOI: 10.1371/journal.pone.0183234

Song C, Li J, Liu J, Liu Q. Simple sensitive rapid detection of Escherichia coli O157:H7 in food samples by label-free immunofluorescence strip sensor. Talanta. 2016 Aug 15;156-157:42-47. DOI: 10.1016/j.talanta.2016.04.054.

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