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Watch the molecular machinery of DNA as it replicated inside a cell

Using these proteins, originally found in jellyfish to make them glow, the team where able to focus laser beams on the brightly lit proteins and track them inside a bacteria that normally lives inside the human gut. This allowed scientists to watch the molecular machinery of DNA as it replicated inside a cell one molecule at a time. It revealed for the first time that only one component of this process, called DnaB helicase, remains stable — like a molecular anchor to the process.

DNA Replication Fork (Wikimedia).

In most cells, whether human or bacterial, a new cell is created after an existing cell divides in two. This means that a copy of the original sequence of genes coded in its DNA must be precisely copied and placed into the new cell.

This is thought to be a process that occurs slowly and methodically at set points in time. New research at the University of York, in collaboration with the University of Oxford and McGill University Canada, however, has now tracked this replication process in real-time and shown that it is far more dynamic than the textbooks suggest, occurring instead through a ‘stuttering-like process’ in short bursts.

Using these proteins, originally found in jellyfish to make them glow, the team where able to focus laser beams on the brightly lit proteins and track them inside a bacteria that normally lives inside the human gut.

This allowed scientists to watch the molecular machinery of DNA as it replicated inside a cell one molecule at a time. It revealed for the first time that only one component of this process, called DnaB helicase, remains stable — like a molecular anchor to the process.

In most cells, whether human or bacterial, a new cell is created after an existing cell divides in two. This means that a copy of the original sequence of genes coded in its DNA must be precisely copied and placed into the new cell.

This is thought to be a process that occurs slowly and methodically at set points in time. New research at the University of York, in collaboration with the University of Oxford and McGill University Canada, however, has now tracked this replication process in real-time and shown that it is far more dynamic than the textbooks suggest, occurring instead through a ‘stuttering-like process’ in short bursts.

The process of DNA replication is fundamental to all life and the way errors in the process are resolved is especially important to human health. Errors can give rise to forms of cancer and become more prevalent in an aging population.

This work will help scientists not only understand more fully the basic building blocks of life but potentially also provides new insights into a range of health conditions as well as even shedding new light on how human aging can give rise to diseases associated with errors in copying the DNA from cell to cell.

Research was conducted using the DNA of Escherichia coli cell, bacteria, but However, the next stage of this research will investigate the same process in more complex cells, ultimately including those from humans.

Reference:

Thomas R Beattie, Nitin Kapadia, Emilien Nicolas, Stephan Uphoff, Adam JM Wollman, Mark C Leake, Rodrigo Reyes-Lamothe. Frequent exchange of the DNA polymerase during bacterial chromosome replication. eLife, 2017; 6 DOI: 10.7554/eLife.21763

The above material provided by University of York. Note that image may be edited for aesthetic reasosn and content may be edited for style and length.

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